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Darstellung der Lebensdauer einer monolithischen CIM® QA Disk. Conditions:
Mobile phase: buffer A: 20 mM Tris-HCl buffer, pH 7.4; buffer B: 20 mM
Tris-HCl buffer + 1M NaCl, pH 7.4; Flow rate: 4 ml/min; Gradient: 0-70 %
buffer B in 35 s; Sample: 1 mg/ml of myoglobin (peak 1), 3 mg/ml of
conalbumin (peak 2) and 4 mg/ml of soybean trypsin inhibitor (peak 3)
dissolved in buffer A; peak 4 is a solvent peak; Injection volume: 20 µl;
Detection: UV at 280 nm. |